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Western blot - VGF antibody (ab69989)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)
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Product Name
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VGF antibody
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See all VGF antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VGF
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Immunogen
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17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human brain tissue lysate
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Cellular localization
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Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Stored in secretory vesicles and then secreted
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Research areas
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Neuroscience >> Neurology process >> Neuroendocrinology >> General neuroendocrinology
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Growth Factors/Hormones >> Hormones
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Relevance
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VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VGF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VGF antibody images, references, products related to ab69989 and other tools.
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VGF antibody images:
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 Western blot - VGF antibody (ab69989)
Lane 1 : VGF antibody (ab69989) at 0.5 µg/ml
Lane 2 : VGF antibody (ab69989) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 67 kDa
Observed band size : 67 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)
ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Search PubMed (MEDLINE) for references to VGF
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VGF antibody - more information
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Customer reviews (feedback) regarding VGF antibody |
Customer FAQs regarding VGF antibody |
Protocols for VGF antibody |
Price and availability of products related to VGF antibody |
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VGF antibody - related products:
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VGF antibody - other tools:
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Contact Abcam with a Technical Enquiry about VGF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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