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Western blot - VGF antibody (ab69989)
Western blot - VGF antibody (ab69989)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)
(enlarge)


Product Name 

VGF antibody

 

See all VGF antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to VGF

Immunogen 

17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human brain tissue lysate

Cellular localization 

Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Stored in secretory vesicles and then secreted

Research areas 

Neuroscience >> Neurology process >> Neuroendocrinology >> General neuroendocrinology
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.

 

Alternative names VGF antibody (ab69989)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

7425    (Human)
381677    (Mouse)
29461    (Rat)

Omim

    

602186    (Human)

SwissProt

    

O15240    (Human)
P20156    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VGF antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for VGF antibody images, references, products related to ab69989 and other tools.

 

VGF antibody images:

Product image  Western blot - VGF antibody (ab69989)

Western blot - VGF antibody (ab69989)

Lane 1 : VGF antibody (ab69989) at 0.5 µg/ml
Lane 2 : VGF antibody (ab69989) at 1 µg/ml

Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate

Lysates/proteins at 15 µg per lane.


Predicted band size : 67 kDa
Observed band size : 67 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab69989)

ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

 
 

Search PubMed (MEDLINE) for references to VGF

 

VGF antibody - more information

Customer reviews (feedback) regarding VGF antibody

Customer FAQs regarding VGF antibody

Protocols for VGF antibody

Price and availability of products related to VGF antibody

 

VGF antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for VGF antibody

ab29449

  

Brain (Human) Cytoplasmic Lysate - adult normal tissue

 

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Other VGF Antibodies

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VGF antibody - other tools:

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