Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Growth Factors/Hormones >> Hormones
Overview
Product name
Anti-VGF antibody
See all VGF products (3) ...
Description
Rabbit polyclonal to VGF
Tested applications
ICC/IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from a internal sequence of human VGF.
Positive control
Human lung carcinoma. Extracts from RAW264.7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/500 - 1/1000.Predicted molecular weight: 67 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Tissue specificity
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
Post-translational
modifications
Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Cellular localization
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
Target information above from: UniProt accessionO15240
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NERP-1 antibody
- NERP-2 antibody
- Nerve growth factor inducible antibody
see all
Database links
- Entrez Gene: 7425 Human
- Entrez Gene: 381677 Mouse
- Entrez Gene: 29461 Rat
- Omim: 602186 Human
- SwissProt: O15240 Human
- SwissProt: P20156 Rat
- Unigene: 587325 Human
Anti-VGF antibody images:
Western blot - VGF antibody (ab74140)
All lanes : Anti-VGF antibody (ab74140) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
Lane 2 : Extracts from RAW264.7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 67 kDa
Observed band size : 67 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VGF antibody (ab74140)
Immunohistochemical analysis of VGF in paraffin embedded human lung carcinoma tissue using ab74140, at a 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunocytochemistry/ Immunofluorescence-VGF antibody(ab74140)
ICC/IF image of ab74140 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74140, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-VGF antibody (ab74140)
ab74140 has not yet been referenced specifically in any publications.
Publishing research using ab74140? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"