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Western blot - VGLL1 antibody (ab77019)
Western blot - VGLL1 antibody (ab77019)
(enlarge)


Western blot - VGLL1 antibody (ab77019)
Western blot - VGLL1 antibody (ab77019)
(enlarge)


Product Name 

VGLL1 antibody

 

See all VGLL1 antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to VGLL1

Immunogen 

Recombinant fragment: MEEMKKTAIR LPKGKQKPIK TEWNSRCVLF TYFQGDISSV VDEHFSRALS NIKSPQELTP SSQSEGVMLK NDDSMSPNQW RYSSPWTKPQ PEVPVTNRAA of human VGLL1 (NP_057351, 1 a.a. ~ 101 a.a) partial recombinant protein with a tag.
BLAST 'MEEMKKTAIR LPKGKQKPIK TEWNSRCVLF TYFQGDISSV VDEHFSRALS NIKSPQELTP SSQSEGVMLK NDDSMSPNQW RYSSPWTKPQ PEVPVTNRAA ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, RecFrag

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

VGLL1 transfected 293T cell lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

VGLL1 is a specific coactivator for the mammalian TEFs. The mammalian TEF and the Drosophila scalloped genes belong to a conserved family of transcriptional factors that possesses a TEA/ATTS DNA binding domain.

 

Alternative names VGLL1 antibody (ab77019)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51442    (Human)

Omim

    

300583    (Human)

SwissProt

    

Q99990    (Human)
Q5H915    (Human)

Unigene

    

496843    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VGLL1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for VGLL1 antibody images, references, products related to ab77019 and other tools.

 

VGLL1 antibody images:

Product image  Western blot - VGLL1 antibody (ab77019)

Western blot - VGLL1 antibody (ab77019)

VGLL1 antibody (ab77019) at 5 µg/ml + immunogen at 0.2 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 29 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)

 

Product image  Western blot - VGLL1 antibody (ab77019)

Western blot - VGLL1 antibody (ab77019)

All lanes : VGLL1 antibody (ab77019) at 5 µg/ml

Lane 1 : VGLL1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 29 kDa
Observed band size : 29 kDa

 
 

Search PubMed (MEDLINE) for references to VGLL1

 

VGLL1 antibody - more information

Customer reviews (feedback) regarding VGLL1 antibody

Customer FAQs regarding VGLL1 antibody

Protocols for VGLL1 antibody

Price and availability of products related to VGLL1 antibody

 

VGLL1 antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for VGLL1 antibody

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

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Alkaline Phosphatase antibody

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FITC antibody

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Biotin antibody

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Phycoerythrin antibody

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Agarose antibody

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15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other VGLL1 Antibodies

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VGLL1 antibody - other tools:

Contact Abcam with a Technical Enquiry about VGLL1 antibody

 

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