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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-VGLL1 antibody
See all VGLL1 products (2) ...
Description
Mouse monoclonal to VGLL1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human, Recombinant Fragment
Immunogen
Recombinant fragment: MEEMKKTAIR LPKGKQKPIK TEWNSRCVLF TYFQGDISSV VDEHFSRALS NIKSPQELTP SSQSEGVMLK NDDSMSPNQW RYSSPWTKPQ PEVPVTNRAA of human VGLL1 (NP_057351, 1 a.a. ~ 101 a.a) partial recombinant protein with a tag.
MEEMKKTAIR LPKGKQKPIK TEWNSRCVLF TYFQGDISSV VDEHFSRALS NIKSPQELTP SSQSEGVMLK NDDSMSPNQW RYSSPWTKPQ PEVPVTNRAA
Positive control
VGLL1 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 29 kDa.
Target
Relevance
VGLL1 is a specific coactivator for the mammalian TEFs. The mammalian TEF and the Drosophila scalloped genes belong to a conserved family of transcriptional factors that possesses a TEA/ATTS DNA binding domain.
Cellular localization
Nuclear
Alternative names
- TDU antibody
- TONDU antibody
- Transcription cofactor vestigial like protein 1 antibody
- Vestigial like 1 antibody
Database links
- Entrez Gene: 51442 Human
- Omim: 300583 Human
- SwissProt: Q5H915 Human
- SwissProt: Q99990 Human
- Unigene: 496843 Human
Anti-VGLL1 antibody images:
Western blot - VGLL1 antibody (ab77019)
Anti-VGLL1 antibody (ab77019) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 29 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Western blot - VGLL1 antibody (ab77019)
All lanes : Anti-VGLL1 antibody (ab77019) at 5 µg/ml
Lane 1 : VGLL1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 29 kDa
Observed band size : 29 kDa
References for Anti-VGLL1 antibody (ab77019)
ab77019 has not yet been referenced specifically in any publications.
Publishing research using ab77019? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"