Anti-VILIP1 antibody (ab190372)
Key features and details
- Chicken polyclonal to VILIP1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Cow, Pig
- Isotype: IgY
Overview
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Product name
Anti-VILIP1 antibody
See all VILIP1 primary antibodies -
Description
Chicken polyclonal to VILIP1 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Pig
Predicted to work with: Chicken, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human VILIP1 aa 1 to the C-terminus. purified from E. coli.
Database link: P62760 -
Positive control
- IHC-P: Human cerebellum tissue. ICC/IF: Adult rat cerebellar cortex cells. WB: Rat brain, mouse brain, pig hippocampus, cow cerebellum lysates and rat brain homogenate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/10000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/1000 - 1/2000.
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IHC-P |
1/5000.
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Notes |
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WB
1/5000 - 1/10000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/1000 - 1/2000. |
IHC-P
1/5000. |
Target
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Function
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. -
Tissue specificity
Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level). -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 396189 Chicken
- Entrez Gene: 282124 Cow
- Entrez Gene: 26950 Mouse
- Entrez Gene: 100173440 Orangutan
- Entrez Gene: 24877 Rat
- SwissProt: P62764 Chicken
- SwissProt: P62763 Cow
- SwissProt: Q4R5F7 Cynomolgus monkey
see all -
Alternative names
- 21 kDa CABP antibody
- Hippocalcin like protein 3 antibody
- Hippocalcin-like protein 3 antibody
see all
Images
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Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to visinin-like protein (VLP1), dilution 1/5000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab190372 labels granule cell membranes as well as synapses of the molecular and granular layers.
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Confocal immunofluorescent analysis of adult rat cerebellar cortex labeling VILIP1 with ab190372 at 1/1000 dilution (green) followed by polyclonal antibody to NF-M (red) and DNA (blue). ML: molecular layer; GC: granule cell layer; PC: Purkinje cells; WM: white matter.
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All lanes : Anti-VILIP1 antibody (ab190372) at 1/10000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Pig hippocampus lysate
Lane 4 : Cow cerebellum lysate
Predicted band size: 22 kDa -
Anti-VILIP1 antibody (ab190372) at 1/5000 dilution + rat brain homogenate
Predicted band size: 22 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab190372 has not yet been referenced specifically in any publications.