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Products:Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
Overview
Product name
Anti-VIP antibody
See all VIP products (7) ...
Description
Rabbit polyclonal to VIP
Specificity
ab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
Tested applications
IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Sheep, Dog, Human, Pig
Immunogen
Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.
Positive control
Human, rat and porcine colon and spinal cord.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-Fr: 1/400 - 1/1600.
IHC-P: 1/400 - 1/1600.
Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
using overnight incubation and indirect immunofluorescence procedure.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
PHM and PHV also cause vasodilation.
Sequence similarities
Belongs to the glucagon family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01282
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC13587 antibody
- Peptide histidine methioninamide 27 antibody
- Peptide histidine valine 42 antibody
see all
Database links
- Entrez Gene: 7432 Human
- Entrez Gene: 117064 Rat
- Omim: 192320 Human
- SwissProt: P01282 Human
- SwissProt: P01284 Pig
- SwissProt: P01283 Rat
- SwissProt: P04565 Sheep
- Unigene: 53973 Human
see all
Anti-VIP antibody images:
Immunohistochemistry (Frozen sections) - VIP antibody (ab22736)
ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.
This image is courtesy of an Abreview submitted by Prof Marcin Arciszewski
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VIP antibody (ab22736)
ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary.
Marcin Arciszewski, Org unknown, Country unknown
References for Anti-VIP antibody (ab22736)
ab22736 has not yet been referenced specifically in any publications.
Publishing research using ab22736? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"