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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VIP antibody, prediluted (ab8795)
(enlarge)
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Product Name
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VIP antibody
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See all VIP antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to VIP
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Immunogen
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Full length native protein (purified) (Human).
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with a 3 kDa vasointestinal peptide (VIP) localized in nerve fibers in the central and peripheral nervous system.
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Tested applications
(see key)
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IHC-FoFr, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
For the localization of VIP in tissue sections.
The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody.
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Positive control
(see definition)
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Bowel.
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Cellular localization
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Secreted
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Research areas
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Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
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Relevance
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Vasoactive Intestinal Peptide (VIP), a 28 amino acid peptide originally isolated from porcine duodenum, is present not only in gastrointestinal tissues but also in neural tissues, possibly as a neurotransmitter, and exhibits a wide variety of biological actions. It belongs to the glucagon family, and in the duodenum is known to stimulate myocardial contractility, cause vasodilation, increase glycogenolysis, lower arterial blood pressure and relax the smooth muscle of trachea, stomach and gall bladder. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome.
This antibody detects ganglion cells in both the superficial and deep plexus of the wall of the small bowel in human. The VIP producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 20mM Tris, 150mM Sodium chloride, 0.5% proprietary proteins. pH 7.6
The buffer does not contain any mammalian proteins.
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this VIP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for VIP antibody images, references, products related to ab8795 and other tools.
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VIP antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VIP antibody, prediluted (ab8795)
Vasoactive intestinal polypeptide immunoreactive cells from a prolactin-secreting tumour. Scale bar represents 100 µm.
This picture was kindly supplied as part of the review submitted by Dr John Classey.
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References for VIP antibody (ab8795)
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This product has been used in:
Nathoo S et al. No relationship between vasoactive intestinal polypeptide expression and headache in pituitary tumours. Acta Neurol Scand 111:317-22 (2005). PubMed: 15819711
If you publish research using ab8795 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to VIP
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VIP antibody - more information
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Customer reviews (feedback) regarding VIP antibody |
Customer FAQs regarding VIP antibody |
Protocols for VIP antibody |
Price and availability of products related to VIP antibody |
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VIP antibody - related products:
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VIP antibody - other tools:
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Contact Abcam with a Technical Enquiry about VIP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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