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Anti-VMA2 antibody [13D11B2] (ab113684)

MSCatalog No. MSZ03

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Overview

Product name

Anti-VMA2 antibody [13D11B2]

Description

Mouse monoclonal [13D11B2] to VMA2

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Saccharomyces cerevisiae

Immunogen

Full length native protein (purified)

Positive control

Yeast membranes

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

13D11B2

Isotype

IgG1

Light chain type

kappa

  • Western blot - VMA2 antibody [13D11B2] (ab113684)Western blot - VMA2 antibody [13D11B2] (ab113684) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab113684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 20 µg/ml.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

Function

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles.

Sequence similarities

Belongs to the ATPase alpha/beta chains family.

Target information above from: UniProt accessionP16140 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • MSZ03 antibody
  • V-ATPase 57 kDa subunit antibody
  • V-ATPase subunit B antibody
  • V-type proton ATPase subunit B antibody
  • Vacuolar proton pump subunit B antibody
  • VATB_YEAST antibody
  • VMA2 antibody
see all

Database links

Anti-VMA2 antibody [13D11B2] images:

  Western blot - VMA2 antibody [13D11B2] (ab113684)

Western blot - VMA2 antibody [13D11B2] (ab113684)

Anti-VMA2 antibody [13D11B2] (ab113684) + Yeast whole cell lysate at 15 µg
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 58 kDa

References for Anti-VMA2 antibody [13D11B2] (ab113684)

ab113684 has not yet been referenced specifically in any publications.

Publishing research using ab113684? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"