![Western blot - VPH1 antibody [10D7A7B2] (ab113683) image](#VPH1-Primary-antibodies-ab113683-2.jpg)
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Product name
Anti-VPH1 antibody [10D7A7B2]
Description
Mouse monoclonal [10D7A7B2] to VPH1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Full length native protein (purified)
Positive control
Yeast membranes
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Clonality
Monoclonal
Clone number
10D7A7B2
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Cofactors, Vitamins / minerals >> Vitamins / minerals
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Alcohol metabolism
Signal Transduction >> Metabolism >> Alcohol metabolism
Signal Transduction >> Metabolism >> Vitamins / Minerals
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Western blot - VPH1 antibody [10D7A7B2] (ab113683)
![Western blot - VPH1 antibody [10D7A7B2] (ab113683) image](/ps/datasheet/images/113/ab113683/VPH1-Primary-antibodies-ab113683-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC/IF: Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.
Target
Function
Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Sequence similarities
Belongs to the V-ATPase 116 kDa subunit family.
Post-translational
modifications
Glycosylated.
Cellular localization
Vacuole membrane.
Target information above from: UniProt accessionP32563
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- vacuolar isoform antibody
- MSZ02 antibody
- V-ATPase 95 kDa subunit antibody
see all
Database links
- Entrez Gene: 854444 Saccharomyces cerevisiae
- SwissProt: P32563 Saccharomyces cerevisiae
Anti-VPH1 antibody [10D7A7B2] images:
Western blot - VPH1 antibody [10D7A7B2] (ab113683)
All lanes : Anti-VPH1 antibody [10D7A7B2] (ab113683)
Lane 1 : Yeast whole cell lysate
Lane 2 : yeast membrane preparation
Lysates/proteins at 15 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 95 kDa
References for Anti-VPH1 antibody [10D7A7B2] (ab113683)
ab113683 has not yet been referenced specifically in any publications.
Publishing research using ab113683? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"