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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-VPRBP antibody
Description
Rabbit polyclonal to VPRBP
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Horse, Cow, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human VPRBP.
(Peptide available as ab86242.)
Positive control
This antibody gave a positive signal in Rat and Mouse Liver Tissue Lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 169 kDa (predicted molecular weight: 169 kDa).
Target
Relevance
VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase.
Cellular localization
Cytoplasmic
Alternative names
- DDB1 and CUL4 associated factor 1 antibody
- DCAF1 antibody
- VPR BP antibody
- Vpr interacting protein antibody
Database links
- Entrez Gene: 9730 Human
- SwissProt: Q9Y4B6 Human
- Unigene: 118738 Human
Anti-VPRBP antibody images:
Western blot - VPRBP antibody (ab75458)
All lanes : Anti-VPRBP antibody (ab75458) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 169 kDa
Observed band size : 169 kDa
Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - VPRBP antibody (ab75458)
ICC/IF image of ab75458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPRBP antibody (ab75458)
IHC image of VPRBP staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75458 at 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-VPRBP antibody (ab75458)
ab75458 has not yet been referenced specifically in any publications.
Publishing research using ab75458? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"