Overview
Product nameVPS25 protein (His tag)See all VPS25 proteins and peptides ...
Protein descriptionRecombinant full length Human VPS25 (amino acids 1-176) with an N terminal His tag (200 amino acids) with the tag); predicted molecular weight 23.3 kDa (NP_115729; UniProt ID = Q9BRG1).
Uniprot accessionQ9BRG1
Molecular weight23.300kDa inclusive of tags
Protein length200 amino acids
Expression hostE. coli
Properties
Purity> 85
% by SDS-PAGE
Purification notesab124321 was purified using conventional chromatography techniques.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride
Concentration information loading...
Sequence notesMGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab124321
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab42500 and ab156230.
Protein info
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Alternative names
Dermal papilla derived protein 9Dermal papilla-derived protein 9DERP9
EAP20ELL associated protein of 20 kDaELL-associated protein of 20 kDaESCRT-II complex subunit VPS25FAP20HGNC:28122hVps25MGC10540Vacuolar protein sorting 25 homolog (S. cerevisiae)Vacuolar protein sorting associated protein 25Vacuolar protein-sorting-associated protein 25VPS25VPS25_HUMAN
see all
FunctionComponent of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
Tissue specificityExpressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.
Sequence similaritiesBelongs to the VPS25 family.
Cellular localizationCytoplasm. Endosome membrane. Nucleus > nucleoplasm. Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
VPS25 protein (His tag) images
References for VPS25 protein (His tag) (ab124321)
ab124321
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - VPS25 protein (His tag) (ab124321)ab124321 (VPS25 protein), at 3 µg, analyzed by 15% SDS-PAGE.