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- Proteins and Peptides
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Product name
Anti-VPS26 antibody
See all VPS26 products (6) ...
Description
Rabbit polyclonal to VPS26
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human VPS26.
(Peptide available as ab242 88.)
Positive control
Human and mouse kidney lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with VPS26 peptide (ab24288).
IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml.
Target
Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Sequence similarities
Belongs to the VPS26 family.
Cellular localization
Cytoplasm. Endosome membrane.
Target information above from: UniProt accessionO75436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- VPS26A antibody
- VPS26A antibody
- FLJ12930 antibody
see all
Database links
- Entrez Gene: 9559 Human
- Entrez Gene: 30930 Mouse
- Entrez Gene: 361846 Rat
- Omim: 605506 Human
- SwissProt: O75436 Human
- SwissProt: P40336 Mouse
- SwissProt: Q6AY86 Rat
- Unigene: 499925 Human
see all
Anti-VPS26 antibody images:
Western blot - VPS26 antibody (ab23892)
All lanes : Anti-VPS26 antibody (ab23892) at 1 µg/ml ab23892 detects a band of ~ 38kDa in Human kidney lysate. This band is quenched by the addition of the immunizing peptide, ab24288. ab23892 also detects a band of the correct size in HEK293 and mouse kidney lysate (data not shown).
Lane 1 : Human kidney lysate
Lane 2 : Human kidney lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 25 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPS26 antibody (ab23892)
Image courtesy of Human Protein Atlas
ab23892 staining VPS26 in Human kidney. The paraffin embedded tissue was incubated with ab23892 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23892 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines
Further results for this antibody can be found at www.proteinatlas.org
Immunocytochemistry/ Immunofluorescence - VPS26 antibody (ab23892)
ICC/IF image of ab23892 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23892, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HepG2 cells.
References for Anti-VPS26 antibody (ab23892)
This product has been referenced in:
- Xia B et al. Modulation of cell adhesion and migration by the histone methyltransferase subunit mDpy-30 and its interacting proteins. PLoS One 5:e11771 (2010). ICC/IF; Human.Read more (PubMed: 20668708) »
- Nisar S et al. Regulation of P2Y1 receptor traffic by sorting Nexin 1 is retromer independent. Traffic 11:508-19 (2010). WB; Human.Read more (PubMed: 20070609) »
See all 4 publications for this product
Publishing research using ab23892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"