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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Overview
Product name
Anti-VPS35 antibody
See all VPS35 products (5) ...
Description
Goat polyclonal to VPS35
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: SPESEGPIYEGLIL, corresponding to C terminal amino acids 783-796 of Human VPS35.
C-SPESEGPI YEGLIL
Positive control
Human Brain lysate. Human testis.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration. (In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.)
WB: Use a concentration of 0.01 - 1 µg/ml.Detects a band of approximately 85 kDa (predicted molecular weight: 91 kDa).(Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa observed in human brain and testis lysates (predicted molecular weight: 91 kDa according to NP_060676).)
ICC/IF: Use at an assay dependent dilution.
Target
Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Tissue specificity
Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
Sequence similarities
Belongs to the VPS35 family.
Cellular localization
Cytoplasm. Membrane.
Target information above from: UniProt accessionQ96QK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp434E1211 antibody
- DKFZp434P1672 antibody
- FLJ10752 antibody
see all
Database links
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
- Entrez Gene: 25479 Rat
- Omim: 606931 Human
- SwissProt: Q96QK1 Human
- SwissProt: Q9EQH3 Mouse
- Unigene: 454528 Human
see all
Anti-VPS35 antibody images:
Western blot - VPS35 antibody (ab10099)
Predicted band size : 91 kDa
ab10099 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VPS35 antibody (ab10099)
ab10099 staining Human Testis by IHC-P. Image A) shows cytoplasmic staining of epithelial cells of the epididymis of the rete testis and image B), some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3µg/ml and B) Citrate buffer pH6 at 10ug/ml, HRP-staining.
References for Anti-VPS35 antibody (ab10099)
This product has been referenced in:
- Kingston Det al. Inhibition of retromer activity by herpesvirus saimiri tip leads to CD4 downregulation and efficient T cell transformation. J Virol 85:10627-38 (2011). WB; Human.Read more (PubMed: 21849449) »
- Scott KLet al. GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Nature 459:1085-90 (2009). ICC/IF; Human.Read more (PubMed: 19553991) »
See all 6 publications for this product
Publishing research using ab10099? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"