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Synthetic peptide: SPESEGPIYEGLIL, corresponding to C terminal amino acids 783-796 of Human VPS35.
The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.
Our Abpromise guarantee covers the use of ab10099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.|
|WB||Use a concentration of 0.01 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 91 kDa).Can be blocked with Human VPS35 peptide (ab23181). Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa observed in human brain and testis lysates (predicted molecular weight: 91 kDa according to NP_060676).|
|ICC/IF||Use at an assay dependent concentration.|
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