Anti-VPS35 antibody (ab57632)

Overview

• Product nameAnti-VPS35 antibodySee all VPS35 primary antibodies ...
• Description
  Mouse monoclonal to VPS35
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment: MECLKKALKI ANQCMDPSLQ VQLFIEILNR YIYFYEKEND AVTIQVLNQL IQKIREDLPN LESSEETEQI NKHFHNTLEH LRLRRESPES EGPIYEGLIL , corresponding to amino acids 697-797 of Human VPS35

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Golgi Proteins

  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Regulation

Applications

Target

• FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
• Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
• Sequence similaritiesBelongs to the VPS35 family.
• Cellular localizationCytoplasm. Membrane.

Target information above from: UniProt accession Q96QK1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100133770 Human
  • Entrez Gene: 55737 Human
  • Entrez Gene: 55737 Human
  • Omim: 601501 Human
  • SwissProt: Q96QK1 Human
  • SwissProt: Q96QK1 Human
  • Unigene: 454528 Human

• Alternative names
  DKFZp434E1211 antibodyDKFZp434P1672 antibodyFLJ10752 antibody

  FLJ13588 antibodyFLJ20388 antibodyhVPS35 antibodyMaternal embryonic 3 antibodyMaternal-embryonic 3 antibodyMEM 3 antibodyMEM3 antibodyPARK17 antibodyTCCCTA00141 antibodyVacuolar protein sorting 35 (yeast) antibodyVacuolar protein sorting 35 antibodyVacuolar protein sorting 35 homolog antibodyVacuolar protein sorting associated protein 35 antibodyVacuolar protein sorting-associated protein 35 antibodyVesicle protein sorting 35 antibodyVPS 35 antibodyVPS35 antibodyVPS35_HUMAN antibody

  see all

Anti-VPS35 antibody images

• 

  Western blot - VPS35 antibody (ab57632)
  Western blot against tagged recombinant protein immunogen using ab57632 VPS35 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
• 

  Flow Cytometry - Anti-VPS35 antibody (ab57632)
  Overlay histogram showing HEK293 cells stained with ab57632 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57632, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.