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Overview

  • Product nameAnti-VPS35 antibody
    See all VPS35 primary antibodies
  • Description
    Mouse monoclonal to VPS35
  • Tested applicationsWB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MECLKKALKI ANQCMDPSLQ VQLFIEILNR YIYFYEKEND AVTIQVLNQL IQKIREDLPN LESSEETEQI NKHFHNTLEH LRLRRESPES EGPIYEGLIL , corresponding to amino acids 697-797 of Human VPS35

Properties

Applications

Our Abpromise guarantee covers the use of ab57632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
  • Sequence similaritiesBelongs to the VPS35 family.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E1211 antibody
    • DKFZp434P1672 antibody
    • FLJ10752 antibody
    • FLJ13588 antibody
    • FLJ20388 antibody
    • hVPS35 antibody
    • Maternal embryonic 3 antibody
    • Maternal-embryonic 3 antibody
    • MEM 3 antibody
    • MEM3 antibody
    • PARK17 antibody
    • TCCCTA00141 antibody
    • Vacuolar protein sorting 35 (yeast) antibody
    • Vacuolar protein sorting 35 antibody
    • Vacuolar protein sorting 35 homolog antibody
    • Vacuolar protein sorting associated protein 35 antibody
    • Vacuolar protein sorting-associated protein 35 antibody
    • Vesicle protein sorting 35 antibody
    • VPS 35 antibody
    • VPS35 antibody
    • VPS35_HUMAN antibody
    see all

Anti-VPS35 antibody images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: VPS35 knockout HAP1 cell lysate (20 µg)

    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: A549 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab57632 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab57632 was shown to specifically react with VPS35 when VPS35 knockout samples were used. Wild-type and VPS35 knockout samples were subjected to SDS-PAGE. ab5732 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot against tagged recombinant protein immunogen using ab57632 VPS35 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • Overlay histogram showing HEK293 cells stained with ab57632 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57632, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-VPS35 antibody (ab57632)

This product has been referenced in:
  • Zavodszky E  et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24819384) »

See 1 Publication for this product

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