Overview

  • Product nameAnti-VPS35 antibodySee all VPS35 primary antibodies ...
  • Description
    Rabbit polyclonal to VPS35
  • Tested applicationsWB, IHC-P, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide from the N terminal region of human VPS35, conjugated to an immunogenic carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab76329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 92 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P Use a concentration of 10 - 50 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 10 - 50 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 22021616

Target

  • FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
  • Sequence similaritiesBelongs to the VPS35 family.
  • Cellular localizationCytoplasm. Membrane.
  • Target information above from: UniProt accession Q96QK1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E1211 antibody
    • DKFZp434P1672 antibody
    • FLJ10752 antibody
    • FLJ13588 antibody
    • FLJ20388 antibody
    • hVPS35 antibody
    • Maternal embryonic 3 antibody
    • Maternal-embryonic 3 antibody
    • MEM 3 antibody
    • MEM3 antibody
    • PARK17 antibody
    • TCCCTA00141 antibody
    • Vacuolar protein sorting 35 (yeast) antibody
    • Vacuolar protein sorting 35 antibody
    • Vacuolar protein sorting 35 homolog antibody
    • Vacuolar protein sorting associated protein 35 antibody
    • Vacuolar protein sorting-associated protein 35 antibody
    • Vesicle protein sorting 35 antibody
    • VPS 35 antibody
    • VPS35 antibody
    • VPS35_HUMAN antibody
    see all

References for Anti-VPS35 antibody (ab76329)

This product has been referenced in:
  • McLeod IX  et al. The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7Ra surface expression. J Immunol 187:5051-61 (2011). ICC/IF, Flow Cyt ; Mouse . Read more (PubMed: 22021616) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"