Overview
- Product nameAnti-VPS35 antibodySee all VPS35 primary antibodies ...
- DescriptionRabbit polyclonal to VPS35
- Tested applicationsWB, IHC-P, IHC-Fr, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide from the N terminal region of human VPS35, conjugated to an immunogenic carrier protein.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 92 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
| IHC-P | IHC-P: Use a concentration of 10 - 50 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IHC-Fr | IHC-Fr: Use a concentration of 10 - 50 µg/ml. |
| Flow Cyt | Flow Cyt: Use at an assay dependent concentration. PubMed: 22021616 |
Target
- FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
- Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
- Sequence similaritiesBelongs to the VPS35 family.
- Cellular localizationCytoplasm. Membrane.
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Database links
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
- Entrez Gene: 25479 Rat
- Omim: 601501 Human
- SwissProt: Q96QK1 Human
- SwissProt: Q96QK1 Human
- SwissProt: Q9EQH3 Mouse
- Unigene: 454528 Human
- Unigene: 296520 Mouse
- Unigene: 391632 Mouse
- Unigene: 161720 Rat
see all
Target information above from: UniProt accession
Q96QK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DKFZp434E1211 antibodyDKFZp434P1672 antibodyFLJ10752 antibody
- FLJ13588 antibodyFLJ20388 antibodyhVPS35 antibodyMaternal embryonic 3 antibodyMaternal-embryonic 3 antibodyMEM 3 antibodyMEM3 antibodyPARK17 antibodyTCCCTA00141 antibodyVacuolar protein sorting 35 (yeast) antibodyVacuolar protein sorting 35 antibodyVacuolar protein sorting 35 homolog antibodyVacuolar protein sorting associated protein 35 antibodyVacuolar protein sorting-associated protein 35 antibodyVesicle protein sorting 35 antibodyVPS 35 antibodyVPS35 antibodyVPS35_HUMAN antibody
see all
References for Anti-VPS35 antibody (ab76329)
This product has been referenced in:
- McLeod IX et al. The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7Ra surface expression. J Immunol 187:5051-61 (2011). ICC/IF, Flow Cyt ; Mouse . Read more (PubMed: 22021616) »
