Anti-VPS35 antibody (ab97545)
Key features and details
- Rabbit polyclonal to VPS35
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VPS35 antibody
See all VPS35 primary antibodies -
Description
Rabbit polyclonal to VPS35 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to a region within amino acids 733-796 (KENDAVTIQV LNQLIQKIRE DLPNLESSEE TEQINKHFHN TLEHLRLRRE SPESEGPIYE GLIL) of Human VPS35 (NP_060676).
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Positive control
- H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 92 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
Target
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Function
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
Tissue specificity
Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. -
Sequence similarities
Belongs to the VPS35 family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 521864 Cow
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
- Entrez Gene: 25479 Rat
- Omim: 601501 Human
- SwissProt: Q2HJG5 Cow
- SwissProt: Q96QK1 Human
see all -
Alternative names
- DKFZp434E1211 antibody
- DKFZp434P1672 antibody
- FLJ10752 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab97545 has been referenced in 20 publications.
- Mishra S et al. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. Cell Mol Life Sci 79:162 (2022). PubMed: 35226190
- Muzio L et al. Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis. Nat Commun 11:3848 (2020). PubMed: 32737286
- Evans AJ et al. Acute inactivation of retromer and ESCPE-1 leads to time-resolved defects in endosomal cargo sorting. J Cell Sci 133:N/A (2020). PubMed: 32747499
- Curnock R & Cullen PJ Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1. J Cell Sci 133:N/A (2020). PubMed: 32843536
- Casanova A et al. A Role for the VPS Retromer in Brucella Intracellular Replication Revealed by Genomewide siRNA Screening. mSphere 4:N/A (2019). PubMed: 31243080