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Overview

  • Product nameAnti-VPS35 antibody
    See all VPS35 primary antibodies
  • Description
    Rabbit polyclonal to VPS35
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 733-796 (KENDAVTIQV LNQLIQKIRE DLPNLESSEE TEQINKHFHN TLEHLRLRRE SPESEGPIYE GLIL) of Human VPS35 (NP_060676).

  • Positive control
    • H1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab97545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.

Target

  • FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
  • Sequence similaritiesBelongs to the VPS35 family.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E1211 antibody
    • DKFZp434P1672 antibody
    • FLJ10752 antibody
    • FLJ13588 antibody
    • FLJ20388 antibody
    • hVPS35 antibody
    • Maternal embryonic 3 antibody
    • Maternal-embryonic 3 antibody
    • MEM 3 antibody
    • MEM3 antibody
    • PARK17 antibody
    • TCCCTA00141 antibody
    • Vacuolar protein sorting 35 (yeast) antibody
    • Vacuolar protein sorting 35 antibody
    • Vacuolar protein sorting 35 homolog antibody
    • Vacuolar protein sorting associated protein 35 antibody
    • Vacuolar protein sorting-associated protein 35 antibody
    • Vesicle protein sorting 35 antibody
    • VPS 35 antibody
    • VPS35 antibody
    • VPS35_HUMAN antibody
    see all

Anti-VPS35 antibody images

  • All lanes : Anti-VPS35 antibody (ab97545) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate
    Lane 5 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 92 kDa

References for Anti-VPS35 antibody (ab97545)

This product has been referenced in:
  • McGough IJ  et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). Read more (PubMed: 25278552) »
  • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . Read more (PubMed: 23563491) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (mouse cortical primary neuron culture DIV14)
Permeabilization Yes - 0.2% Triton X-100
Specification mouse cortical primary neuron culture DIV14
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Julie Nys

Verified customer

Submitted Nov 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"