Overview
- Product nameAnti-VPS35 antibodySee all VPS35 primary antibodies ...
- DescriptionRabbit polyclonal to VPS35
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow - Immunogen
Synthetic peptide corresponding to a region within amino acids 733-796 (KENDAVTIQV LNQLIQKIRE DLPNLESSEE TEQINKHFHN TLEHLRLRRE SPESEGPIYE GLIL) of Human VPS35 (NP_060676).
- Positive controlH1299, HeLa S3, HepG2, MOLT4 and Raji cell lysates
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/3000. Predicted molecular weight: 92 kDa. |
Target
- FunctionEssential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
- Tissue specificityUbiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.
- Sequence similaritiesBelongs to the VPS35 family.
- Cellular localizationCytoplasm. Membrane.
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Database links
- Entrez Gene: 521864 Cow
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
- Entrez Gene: 25479 Rat
- Omim: 601501 Human
- SwissProt: Q2HJG5 Cow
- SwissProt: Q96QK1 Human
- SwissProt: Q96QK1 Human
- SwissProt: Q9EQH3 Mouse
- Unigene: 454528 Human
- Unigene: 296520 Mouse
- Unigene: 391632 Mouse
- Unigene: 161720 Rat
see all
Target information above from: UniProt accession
Q96QK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DKFZp434E1211 antibodyDKFZp434P1672 antibodyFLJ10752 antibody
- FLJ13588 antibodyFLJ20388 antibodyhVPS35 antibodyMaternal embryonic 3 antibodyMaternal-embryonic 3 antibodyMEM 3 antibodyMEM3 antibodyPARK17 antibodyTCCCTA00141 antibodyVacuolar protein sorting 35 (yeast) antibodyVacuolar protein sorting 35 antibodyVacuolar protein sorting 35 homolog antibodyVacuolar protein sorting associated protein 35 antibodyVacuolar protein sorting-associated protein 35 antibodyVesicle protein sorting 35 antibodyVPS 35 antibodyVPS35 antibodyVPS35_HUMAN antibody
see all
Anti-VPS35 antibody images
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Western blot - VPS35 antibody (ab97545)All lanes : Anti-VPS35 antibody (ab97545) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lane 5 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 92 kDa
References for Anti-VPS35 antibody (ab97545)
ab97545 has not yet been referenced specifically in any publications.
