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Product name
Anti-VR1 antibody
See all VR1 products (25) ...
Description
Rabbit polyclonal to VR1
Specificity
This antibody has been optimised on rat tissue. We recommend ab31895 for detection of VR1 in mouse tissue.
Tested applications
WB, ICC, IHC (PFA fixed)more details
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse, Non Human Primates
Immunogen
Synthetic peptide: RASLDSEESESPPQENSC, corresponding to amino-terminus of Rat VR1, amino acids 4-21.
RASLDSEESESPPQENSC
Positive control
ICC: VR1 transfected cells. WB: dorsal root ganglion cell lysate IHC: dorsal root ganglion (perfusion fixed)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole Serum
Purity
Whole antiserum
Primary antibody notes
Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Somatosensory system >> Nociception
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
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Immunohistochemistry (PFA perfusion fixed frozen sections) - VR1 antibody (ab10296)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 100.7 kDa.(see Guo et al reference).
ICC
ICC: 1/1000.ICC: 1/1000.
IHC (PFA fixed): 1/1000. (Perfusion fixed free floating) (see Guo et al reference).
Target
Function
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
Tissue specificity
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats.
Domain
The association domain (AD) is necessary for self-association.
Post-translational
modifications
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
Target information above from: UniProt accessionQ8NER1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
Database links
- Entrez Gene: 193034 Mouse
- Entrez Gene: 83810 Rat
- SwissProt: Q704Y3 Mouse
- SwissProt: O35433 Rat
- Unigene: 447485 Mouse
- Unigene: 3073 Rat
Anti-VR1 antibody images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - VR1 antibody (ab10296)
VR1 staining in the rat dorsal root ganglion using ab10296.
References for Anti-VR1 antibody (ab10296)
This product has been referenced in:
- van den Wijngaard RM et al. Essential role for TRPV1 in stress-induced (mast cell-dependent) colonic hypersensitivity in maternally separated rats. Neurogastroenterol Motil 21:1107-e94 (2009). IHC-FoFr; Rat.Read more (PubMed: 19523146) »
- Chen W et al. Immortalization and characterization of a nociceptive dorsal root ganglion sensory neuronal line. J Peripher Nerv Syst 12:121-30 (2007).Read more (PubMed: 17565537) »
See all 3 publications for this product
Publishing research using ab10296? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"