Verotoxin 2 beta antibody [257] (ab20623)

Product Name 

Verotoxin 2 beta antibody [257]

Product type 

Primary antibodies

Description 

Mouse monoclonal [257] to Verotoxin 2 beta

Immunogen 

Purified toxoid.

Reacts with 
(species key)

N/A

Specificity 

Specific for Verotoxin II - B subunit (SLT-2b). Neutralising antibody.

Tested applications 
(see key)

ELISA

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20 - 1/200.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli

Relevance 

Verotoxins are produced by certain enterohaemorrhagic strains of Escherichia coli. Serotype O157:H7 has been the strain most commonly associated with large food bourne outbreaks and has been implicated in a wide spectrum of diseases including blood diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome. The main sources of infection are contaminated foods of animal origin such as meat and dairy products.
Verotoxins, also called Shiga like toxins( SLT) because of their similarity to toxins produced by Shigella dysenteriae, are classified into two main categories: verotoxin 1 (VT1, SLT1, Stx1) and 2 (VT2, SLT2, Stx2). Verotoxin production is the most common criteria for the detection of this group of bacteria rather than tests for the cytopathic effect on Vero cells that gave the toxins their name.
Verotoxins contain a single A subunit and multiple copies of a B subunit.
The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

257

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 10mM PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

90% pure from ascites.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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