Recombinant Anti-USO1 antibody [EPR2140Y] (ab68137)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2140Y] to USO1
- Suitable for: WB
- Reacts with: Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-USO1 antibody [EPR2140Y]
See all USO1 primary antibodies -
Description
Rabbit monoclonal [EPR2140Y] to USO1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human USO1 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa cell lysate; 293T cell lysate; HepG2 cell lysate; Cos cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2140Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
Target
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Function
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. -
Sequence similarities
Belongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats. -
Domain
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain. -
Post-translational
modificationsPhosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. -
Cellular localization
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. - Information by UniProt
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Database links
- Entrez Gene: 8615 Human
- Entrez Gene: 56041 Mouse
- Entrez Gene: 56042 Rat
- Omim: 603344 Human
- SwissProt: O60763 Human
- SwissProt: Q9Z1Z0 Mouse
- SwissProt: P41542 Rat
- Unigene: 292689 Human
see all -
Alternative names
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
see all
Images
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All lanes : Anti-USO1 antibody [EPR2140Y] (ab68137) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Cos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 108 kDa
Observed band size: 108 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab68137 has been referenced in 1 publication.
- Jin Y & Dai Z USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells. Biomed Pharmacother 78:264-71 (2016). WB ; Human . PubMed: 26898451