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Western blot - Vesicle docking protein p115 antibody (ab32796)

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Product Name
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Vesicle docking protein p115 antibody
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See all Vesicle docking protein p115 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Vesicle docking protein p115
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Immunogen
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Synthetic peptide: MNFLRGVMGG QSAGPQH, corresponding to amino acids 1-17 of Rat Vesicle docking protein p115 (Peptide available as ab39822.) BLAST 'MNFLRGVMGG QSAGPQH' with or 
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu, Cow (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/500. Detects a band of approximately 115 kDa (predicted molecular weight: 107 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Liver samples (Rat and Mouse)
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Cellular localization
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Peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase.
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
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Relevance
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p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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This antibody was purified by peptide affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vesicle docking protein p115 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Vesicle docking protein p115 antibody images, references, products related to ab32796 and other tools.
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Vesicle docking protein p115 antibody images:
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Western blot - Vesicle docking protein p115 antibody (ab32796)
Vesicle docking protein p115 antibody (ab32796) at 1/500 dilution + rat liver at 50 µg
Secondary anti-rabbit HRP
Predicted band size : 107 kDa Observed band size : 115 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Vesicle docking protein p115
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Vesicle docking protein p115 antibody - more information
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