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Anti-Vesicle docking protein p115 antibody (ab40822) at 1 µg/ml + Human kidney lysate - 35µg protein in RIPA buffer
Secondary
anti-goat-HRP at 1/3000 dilution
Predicted band size : 108 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
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