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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Overview
Product name
Anti-Vesicle docking protein p115 antibody
See all Vesicle docking protein p115 products (6) ...
Description
Goat polyclonal to Vesicle docking protein p115
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-DESEDPGKDLDH, corresponding to C terminal amino acids 950-961 of Human Vesicle docking protein p115
C-DESEDPGK DLDH
Positive control
Human kidney lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
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Western blot - Vesicle docking protein p115 antibody (ab40822)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).
Target
Function
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Sequence similarities
Belongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats.
Domain
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Post-translational
modifications
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cellular localization
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
Target information above from: UniProt accessionO60763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
see all
Database links
- Entrez Gene: 8615 Human
- Omim: 603344 Human
- SwissProt: O60763 Human
- SwissProt: Q8N592 Human
- Unigene: 292689 Human
Anti-Vesicle docking protein p115 antibody images:
Western blot - Vesicle docking protein p115 antibody (ab40822)
Anti-Vesicle docking protein p115 antibody (ab40822) at 1 µg/ml + Human kidney lysate - 35µg protein in RIPA buffer
Secondary
anti-goat-HRP at 1/3000 dilution
Predicted band size : 108 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
References for Anti-Vesicle docking protein p115 antibody (ab40822)
ab40822 has not yet been referenced specifically in any publications.
Publishing research using ab40822? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"