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Western blot - Vesicle docking protein p115 antibody (ab40822)
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Product Name
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Vesicle docking protein p115 antibody
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See all Vesicle docking protein p115 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Vesicle docking protein p115
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Immunogen
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Synthetic peptide: C-DESEDPGKDLDH, corresponding to C terminal amino acids 950-961 of Human Vesicle docking protein p115
BLAST 'C-DESEDPGKDLDH' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Detection limit dilution - 1/2000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Kidney lysates.
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Cellular localization
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Peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase.
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
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Relevance
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p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments.
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Vesicle docking protein p115 antibody (ab40822) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vesicle docking protein p115 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Vesicle docking protein p115 antibody images, references, products related to ab40822 and other tools.
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Vesicle docking protein p115 antibody images:
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 Western blot - Vesicle docking protein p115 antibody (ab40822)
Vesicle docking protein p115 antibody (ab40822) at 1 µg/ml + Human Kidney lysate - 35µg protein in RIPA buffer
Secondary
anti-goat-HRP at 1/3000 dilution
Predicted band size : 108 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Vesicle docking protein p115
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Vesicle docking protein p115 antibody - more information
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Customer reviews (feedback) regarding Vesicle docking protein p115 antibody |
Customer FAQs regarding Vesicle docking protein p115 antibody |
Protocols for Vesicle docking protein p115 antibody |
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Vesicle docking protein p115 antibody - related products:
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Vesicle docking protein p115 antibody - other tools:
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