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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31544? Please let us know so that we can cite the reference in this datasheet
ab31544 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
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