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Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
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