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Read our guarantee »Products:Neuroscience >> Neurotransmitter >> Acetylcholine
Anti-Vesicular Acetylcholine Transporter antibody
See all Vesicular Acetylcholine Transporter products (8) ...
Sheep polyclonal to Vesicular Acetylcholine Transporter
Ab31544 is specific to vesicular acetylcholine transporter.
IHC - Wholemount, WBmore details
Reacts with
Rat, Guinea pig
Predicted to work with
Mouse, Dog, Human, Chimpanzee, Macaque Monkey
Synthetic peptide: C-TRSRSERDVLLDEPPQGLYDAVRLRE, corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.
C-TRSRSERD VLLDEPPQGL YDAVRLRE
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: Whole serum
Whole antiserum
Polyclonal
IgG
Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology
Neuroscience >> Neurotransmitter >> Acetylcholine
Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
(enlarge)
Our Abpromise guarantee covers the use of ab31544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wmt: Use at an assay dependent concentration. (Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Involved in acetylcholine transport into synaptic vesicles.
Peripheral and central cholinergic nervous systems.
Belongs to the major facilitator superfamily. Vesicular transporter family.
Membrane.
Target information above from: UniProt accessionQ16572
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)

Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
ab31544 has not yet been referenced specifically in any publications.
Publishing research using ab31544? Please let us know so that we can cite the reference in this datasheet
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Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
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