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Products:Neuroscience >> Neurotransmitter >> Acetylcholine
Overview
Product name
Anti-Vesicular Acetylcholine Transporter antibody
See all Vesicular Acetylcholine Transporter products (8) ...
Description
Sheep polyclonal to Vesicular Acetylcholine Transporter
Specificity
Ab31544 is specific to vesicular acetylcholine transporter.
Tested applications
IHC - Wholemount, WBmore details
Cross reactivity
Reacts with
Rat, Guinea pig
Predicted to work with
Mouse, Dog, Human, Chimpanzee, Macaque Monkey
Immunogen
Synthetic peptide: C-TRSRSERDVLLDEPPQGLYDAVRLRE, corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.
C-TRSRSERD VLLDEPPQGL YDAVRLRE
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology
Neuroscience >> Neurotransmitter >> Acetylcholine
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Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wmt: Use at an assay dependent concentration. (Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.)
WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Target
Function
Involved in acetylcholine transport into synaptic vesicles.
Tissue specificity
Peripheral and central cholinergic nervous systems.
Sequence similarities
Belongs to the major facilitator superfamily. Vesicular transporter family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ16572
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC12716 antibody
- rVAT antibody
- SLC18A3 antibody
see all
Database links
- Entrez Gene: 6572 Human
- Entrez Gene: 20508 Mouse
- Entrez Gene: 60422 Rat
- Omim: 600336 Human
- SwissProt: Q16572 Human
- SwissProt: O35304 Mouse
- SwissProt: Q3TYJ1 Mouse
- SwissProt: Q62666 Rat
see all
Anti-Vesicular Acetylcholine Transporter antibody images:
Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
References for Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
ab31544 has not yet been referenced specifically in any publications.
Publishing research using ab31544? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"