Vesicular Acetylcholine Transporter antibody (ab34852)

Product Name 

Vesicular Acetylcholine Transporter antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Vesicular Acetylcholine Transporter

Immunogen 

Synthetic 20 amino acid sequence peptide from the rat Vesicular Acetylcholine Transporter protein, conjugated with thyroglobulin using a Glutaraldehyde linker.

Reacts with 

Ms, Rat

Does not react with 
(species key)

Cat, Gpig, Rb

Specificity 

This antibody recognizes Vesicular Acetylcholine Transporter. It does not react with the vesicular monoamine transporter (VMAT).

Tested applications 
(see key)

ICC/IF, IHC-FoFr, IHC-P

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/50 - 1/200.
IHC-F: 1/200 - 1/1600.
Note: tissues can be fixed with 4% paraformaldehyde or 4% paraformaldehyde and 0.1% picric acid in 160 mM sodium phosphate buffer.

Staining pattern: Gives pattern expected for cholinergic neurons and neuronal pathways in sections of the central and peripheral nervous system.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Plasma membrane

Research areas 

Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology
Neuroscience >> Neurotransmitter >> Acetylcholine

Relevance 

Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate.
VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Ab34852 was purified by Blue Sepharose chromatography.

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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