Vesicular Acetylcholine Transporter antibody (ab62140)

Product Name 

Vesicular Acetylcholine Transporter antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Vesicular Acetylcholine Transporter

Immunogen 

A synthetic peptide corresponding to a part of human, mouse and rat Vesicular Acetylcholine Transporter conjugated to an immunogenic carrier protein.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab62140 appears to be specific for Vesicular Acetylcholine Transporter.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Predicted molecular weight: 59 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Plasma membrane

Research areas 

Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology
Neuroscience >> Neurotransmitter >> Acetylcholine

Relevance 

Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate.
VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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