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Product Name
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Vesicular Acetylcholine Transporter antibody
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See all Vesicular Acetylcholine Transporter antibodies (8)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Vesicular Acetylcholine Transporter
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Immunogen
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A synthetic peptide corresponding to a part of human, mouse and rat Vesicular Acetylcholine Transporter conjugated to an immunogenic carrier protein.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab62140 appears to be specific for Vesicular Acetylcholine Transporter.
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Tested applications
(see key)
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended. IHC-Fr: 1/300 - 1/3000. WB: 1/300 - 1/3000. Predicted molecular weight: 59 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Plasma membrane
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Research areas
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Neuroscience >> Neuropharmacology >> Molecular Neuropharmacology Neuroscience >> Neurotransmitter >> Acetylcholine
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Relevance
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Vesicular Acetylcholine Transporter (VAChT), (~70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer’s disease and other nervous system disorders.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vesicular Acetylcholine Transporter antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Vesicular Acetylcholine Transporter
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