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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab68984? Please let us know so that we can cite the reference in this datasheet
ab68984 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vesicular Acetylcholine Transporter antibody (ab68984)
ab68984 at a 1/200 dilution staining Vesicular Acetylcholine Transporter in rat spinal cord tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Incubated overnight at 4°C, developed with DAB/Ni and counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Vesicular Acetylcholine Transporter antibody (ab68984)
ICC/IF image of ab68984 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68984, 1/100 dilution) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Vesicular Acetylcholine Transporter antibody (ab68984)
All lanes : Anti-Vesicular Acetylcholine Transporter antibody (ab68984) at 1/500 dilution
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
Vesicular Acetylcholine Transporter contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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