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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-Vimentin antibody - Neural Stem Cell Marker
See all Vimentin products (41) ...
Description
Rabbit polyclonal to Vimentin - Neural Stem Cell Marker
Tested applications
IHC-P, WB, ICC/IF, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Cow, Human
Predicted to work with
Rat, Sheep, Chicken, Pig, Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human Vimentin.
(Peptide available as ab46156)
Positive control
ab45939 gave a positive result in the following whole cell lysates: Hela, Jurkat and HEK293. In addition, ab45939 gave a negative result in Ramos whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Vimentin
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/700Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF: Use a concentration of 1 µg/ml
IHC-Fr: 1/50
Target
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments.
Target information above from: UniProt accessionP08670
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Vimentin is found in connective tissue and in the cytoskeleton.
Alternative names
- FLJ36605 antibody
- VIM antibody
- VIME_HUMAN antibody
- vimentin antibody
Database links
- Entrez Gene: 420519 Chicken
- Entrez Gene: 493952 Chimpanzee
- Entrez Gene: 280955 Cow
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 407747 Pig
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
see all
Anti-Vimentin antibody - Neural Stem Cell Marker images:
Western blot - Vimentin antibody - Neural Stem Cell Marker (ab45939)
All lanes : Anti-Vimentin antibody - Neural Stem Cell Marker (ab45939) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Western blot - Vimentin antibody - Neural Stem Cell Marker (ab45939)
All lanes : Anti-Vimentin antibody - Neural Stem Cell Marker (ab45939) at 1 µg/ml
Lane 1 : Vimentin protein (Human) (ab73843) at 0.1 µg
Lane 2 : Vimentin protein (Human) (ab73843) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 54 kDa
Exposure time : 10 seconds
Ab45939 recognizes full length recombinant Human vimentin (ab73843) which has an expected molecular weight of 54 kDa.
Immunocytochemistry/ Immunofluorescence - Vimentin antibody - Neural Stem Cell Marker (ab45939)
ICC/IF image of ab45939 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45939, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody - Neural Stem Cell Marker (ab45939)
Immunohistochemical detection (formaldehyde/paraffin-embedded sections) of vimentin protein using vimentin antibody - Neural Stem Cell Marker (ab45939) on human ovarian carcinoma sections. Antigen retrieval step:Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. Ab45939 was used at 1/700 for 1 hour. Secondary Antibody: Biotin-conjugated anti rabbit IG (1/300).
Carl Hobbs, CARD, KCL, UK
Immunohistochemistry (Frozen sections) - Vimentin antibody - Neural Stem Cell Marker (ab45939)
ab45939 staining Vimentin in human squamous cell carcinoma tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with formaldehyde and blocked with 4% milk for 20 minutes at 22°C. The sample was incubated with primary antibody (1/50 in milk) at 4°C for 18 hours. A Biotin-conjugated Goat polyclonal to rabbit IgG (1/200) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody - Neural Stem Cell Marker (ab45939)
ab45939 staining Vimentin in murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/2100 dilution for 18 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.
Image courtesy of Mike Forbes by Abreview.
Immunocytochemistry/ Immunofluorescence - Vimentin antibody - Neural Stem Cell Marker (ab45939)
ICC/IF image of ab45939 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45939, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Vimentin antibody - Neural Stem Cell Marker (ab45939)
This product has been referenced in:
- Bargagna-Mohan Pet al. Corneal antifibrotic switch identified in genetic and pharmacological deficiency of vimentin. J Biol Chem 287:989-1006 (2012). IHC-Fr; Mouse.Read more (PubMed: 22117063) »
- Johnson JRet al. Chronic respiratory aeroallergen exposure in mice induces epithelial-mesenchymal transition in the large airways. PLoS One 6:e16175 (2011). IHC-Fr; Mouse.Read more (PubMed: 21283768) »
See all 7 publications for this product
Publishing research using ab45939? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"