Western blot - anti-Vimentin antibody (ab8978)

One-dimensional immunoblot of BHK-cells incubated with (a) monoclonal desmin antibody, ab8976 and (b) re-incubated with the monoclonal vimentin antibody, ab8978.

Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

Ab8978 vimentin staining of a tonsilar lymphoma. Note that the epithelium (at the left) is negative.

Immunofluorescence - Vimentin antibody [RV202] (ab8978)

Ab8978 vimentin immunofluorescent staining of cultured bovine lens epithelial cells

Immunocytochemistry/ Immunofluorescence - anti-Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

ab8978 staining Vimentin in bovine chromospheres by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and permeabilized in 0.3% Triton X-100. The primary antibody (1/500) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor^® 568-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary.  

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

ab8978 staining Vimentin in human immortalized primary epithelial primary cells by ICC/IF (Immunocytochemistry/ Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with PBS and 0.5% Triton ×100 and blocking with 0.1% BSA + 10% Goat Serum at 25⁰C for 30 minutes was performed. Samples were incubated with primary antibody (1/250: in PBS, 0.1% BSA and 10% Goat Serum) for 12 hours at 4°C. An Alexa Fluor^®594-conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

This image is a courtesy of Anonymous Abreview

Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

ab8978 staining Vimentin in human lung tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 5% commercially available blocking agent was performed at 37⁰C  for 15 minutes. The sample was incubated with primary antibody (1/250) at 37⁰C for 1 hour. A HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

This image is a courtesy of Anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

ab8978 staining vimentin in human pancreatic adenocarcinoma cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.2% Triton X prior to blocking in 3% BSA for 30 minutes at 24ºC. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 21ºC. Alexa fluor® 488 mouse polyclonal to mouse Ig, diluted 1/300 was used as the secondary antibody

This image is courtesy of an Abreview submitted by Jennifer Dembinski

Flow Cytometry - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

Overlay histogram showing HeLa cells stained with ab8978 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8978, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This anti-Vimentin antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton X-100® used under the same conditions.

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab8978 reacts with in connective tissue cells and bloodvessels. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

ab8978 staining Vimentin - Neural Stem Cell Marker in Human Colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized in 0.1% Triton and blocked with 0.25% serum free protein blocker for 20 minutes at 28ºC. Samples were incubated with primary antibody (1/100 in antibody diluent) for 2 hours at 28ºC. ab6785 Goat polyclonal anti-Mouse IgG - H&L (FITC) (1/800) was used as the secondary antibody. Nuclei were counterstained with propidium iodide.

This image is courtesy of an Abreview submitted by Dr J. Chai