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Product Name
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Vimentin antibody [V9]
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See all Vimentin antibodies (34)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [V9] to Vimentin
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Immunogen
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Porcine Lens
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Reacts with
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Hu, Rat, Cat, Chk, Dog, Hrs, Pig
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Does not react with
(species key)
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Ms
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Specificity
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The antibody stains the 57 kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes.
Does not react with GFAP, neurofilamen or desmin.
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Tested applications
(see key)
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Flow Cyt, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
IHC-F: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100. Perform enzymatic(trypsin) antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/300.
WB: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma
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Cellular localization
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Cytoplasmic
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Vimentin
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
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Vimentin antibody [V9] (ab8069) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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V9
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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1 ml purified supernatant in PBS with approximately 30 µg antibody/ml and 20mM sodium azide and 1% BSA. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.030 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vimentin antibody [V9] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Vimentin antibody [V9] (ab8069)
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This product has been used in: (two most recent references)
Sheyn D et al. Nonvirally engineered porcine adipose tissue-derived stem cells: use in posterior spinal fusion. Stem Cells 26:1056-64 (2008). IHC-P; Pig. PubMed: 18218819
Mark Kristiansen et al Disease Related prion protein form aggreasomes in Neuronal Cells leading to Caspase Activiation and Apoptosis. The Journal of Biological Chemistry 280:38851-38861 (2005).
If you publish research using ab8069 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Vimentin
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