Overview

  • Product nameAnti-Vimentin antibody [VI-01]See all Vimentin primary antibodies ...
  • Description
    Mouse monoclonal [VI-01] to Vimentin
  • SpecificityBesides reactivity with vimentin, this antibody also cross-reacts with smooth muscle desmin. A positive reaction is also found on thyroid folicular cells and several mesothelial cells.
  • Tested applicationsSandwich ELISA, ELISA, ICC, WB, ICC/IF, IHC-P, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Human, Pig
    Predicted to work with: a wide range of other species, all Mammals
  • Immunogen

    Pellet of pig brain cold stable proteins after depolymerization of microtubules.

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab7752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [VI-RE/1] to Vimentin (ab3974).
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
IP Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
  • Cellular localizationCytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • vimentin antibody
    see all

Anti-Vimentin antibody [VI-01] images

  • Ab7752 staining human kidney tissue. Staining can be observed in the cytoplasm of cells.
    Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Image from Ermakova S et al, J Biol Chem 280:16882-90 (2005), Fig 4.

  • ICC/IF image of ab7752 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7752, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab7752 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100® for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7752, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100® used under the same conditions.

References for Anti-Vimentin antibody [VI-01] (ab7752)

This product has been referenced in:
  • Xu C  et al. Stromal-epithelial interactions modulate cross-talk between prolactin receptor and HER2/Neu in breast cancer. Breast Cancer Res Treat 134:157-69 (2012). Read more (PubMed: 22270933) »
  • Acampora KB  et al. Increased synthetic phenotype behavior of smooth muscle cells in response to in vitro balloon angioplasty injury model. Ann Vasc Surg 24:116-26 (2010). Read more (PubMed: 19781909) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us.

The protocol we have for GMA staining is as follows;

Place biopsy immediately in ice cold acetone containing protease inhibitors
Fix overnight -20°C
Replace fixative with acetone (room tem...

Read More

Thank you for your enquiry. Ab7752 and ab3974 can be used together in a sandwich ELISA. As both are unconjugated, you will have to use a secondary antibody for detection, just make sure that the secondary antibody does not cross-react with the coating ...

Read More

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with these two antibodies. For the Vimentin antibody, ab7752, we have not received any other complaints about the use of it in Western blotting, and it sounds that perh...

Read More

I have heard back from the originator of this antibody and have been informed that the antibody had not been tested in IP and our datasheet was wrong. I apologise for this error. Our datasheet has been updated and IP removed as a recommended applicatio...

Read More

I have heard back from the originator of this antibody and have been informed that the antibody had not been tested in IP and our datasheet was wrong. I apologise for this error. Our datasheet has been updated and IP removed as a recommended applicatio...

Read More

I have heard back from the originator of this antibody and have been informed that the antibody had not been tested in IP and our datasheet was wrong. I apologise for this error. Our datasheet has been updated and IP removed as a recommended applicatio...

Read More

Thank you for your enquiry. I would suggest trying a 1:50-1:200 titration range and optimizing from there based on your results. Ab7752 has not been tested to our knowledge in IHC-frozen sections, and we would love your feedback. Please let us know...

Read More

We can confirm that IgM is not pulled down well by Protein A beads. We recommend Goat anti Mouse IgM (or polyvalent Ig, or anti-heavy chain) beads. We hope this information helps, please do not hesitate to contac tus if you need further advice,

This antibody has not been specifically tested on rat nervous tissue. However, given the conservation of this molecule, we would expect it to work on rat vimentin

Given the conservation of this molecule, we would expect it to work on mouse vimentin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"