Overview

  • Product nameAnti-Vimentin antibody [Vim 3B4]See all Vimentin primary antibodies ...
  • Description
    Mouse monoclonal [Vim 3B4] to Vimentin
  • SpecificityAb28028 recognises Vimentin
  • Tested applicationsWB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
  • Immunogen

    Full length native protein (purified): Vimentin isolated from bovine eye lens.

  • EpitopeAb28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.
  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab28028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 54 kDa.
IHC-Fr 1/100 - 1/200. The antibody can be used for labeling acetone fixed, frozen sections.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • vimentin antibody
    see all

Anti-Vimentin antibody [Vim 3B4] images

  • ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  •  ab28028 at 1/200 dilution staining vimentin in human lung cancer cells. The Vimentin staining is shown in red. The cells were counterstained with DAPI. Please see accompanying abreview for additional information.

    See Abreview

  • ab28028 staining Vimentin in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton x100. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®488-conjugated donkey monoclonal to mouse IgG (ab150105) was used at dilution at 1/500 as secondary antibody.

    See Abreview

References for Anti-Vimentin antibody [Vim 3B4] (ab28028)

This product has been referenced in:
  • Laimer D  et al. PDGFR blockade is a rational and effective therapy for NPM-ALK-driven lymphomas. Nat Med 18:1699-704 (2012). Human . Read more (PubMed: 23064464) »
  • Dukic-Stefanovic S  et al. Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions. PLoS One 7:e52567 (2012). IHC-Fr ; Human . Read more (PubMed: 23285091) »

See all 8 Publications for this product

Product Wall

Merci de nous avoir contactés.

L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration ...

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We have 4, FITC conjugated anti Glycophorin A antibodies.

http://www.abcam.com/Glycophorin-A-antibody-YTH89-1-FITC-ab28082.html
http://www.abcam.com/Glycophorin-A-antibody-FITC-ab112201.html
http:/...

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Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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Thank you for contacting us and your interest in our products.


We have several antibodies directed against epithelial membrane antigen (also known as MUC1), however only one has been teated in all the applications you wish to use it for...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 1% TX-100
Username

Abcam user community

Verified customer

Submitted Oct 16 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung cancer)
Specification Lung cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Da Zhu

Verified customer

Submitted Mar 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"