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Anti-Vimentin antibody [Vim 3B4] (ab28028)

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Overview

Product name

Anti-Vimentin antibody [Vim 3B4]
See all Vimentin products (41) ...

Description

Mouse monoclonal [Vim 3B4] to Vimentin

Specificity

Ab28028 recognises Vimentin

Tested applications

ICC/IF, IP, WB, ICC, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis

Immunogen

Full length native protein (purified): Vimentin isolated from bovine eye lens.

Epitope

Ab28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.

Positive control

Human osteogenic sarcoma 4-998 cells for IP, cytoskeletal proteins from Xenopus laevis kidney epithelial cells and erythrocytes for WB.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 15mM Sodium Azide.
Constituents: Cell culture supernatant dialysed against 50mM Tris HCl. pH 7.2.

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

Vim 3B4

Isotype

IgG2a

Research areas

Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Vimentin
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [Vim 3B4] (ab28028)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [Vim 3B4] (ab28028) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Tissue specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational
modifications

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments.

Target information above from: UniProt accessionP08670 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Form

Vimentin is found in connective tissue and in the cytoskeleton.

Alternative names

  • FLJ36605 antibody
  • VIM antibody
  • VIME_HUMAN antibody
  • vimentin antibody

Database links

see all

Anti-Vimentin antibody [Vim 3B4] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [Vim 3B4] (ab28028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vimentin antibody [Vim 3B4] (ab28028)

ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)

Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)

ab28028 at 1/200 dilution staining vimentin in human lung cancer cells. The Vimentin staining is shown in red. The cells were counterstained with DAPI. Please see accompanying abreview for additional information.

This image is courtesy of an Abreview submitted by Dr Da Zhu

See Abreview

  Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)

Immunocytochemistry/ Immunofluorescence - Vimentin antibody [Vim 3B4] (ab28028)

ab28028 staining Vimentin in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton x100. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®488-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

This image is a courtesy of Anonymous Abreview

See Abreview

References for Anti-Vimentin antibody [Vim 3B4] (ab28028)

This product has been referenced in:

  • Delavalle PYet al. Hepatocyte-derived cultured cells with unusual cytoplasmic keratin-rich spheroid bodies. Exp Cell Res : (2011). ICC/IF; Human.Read more (PubMed: 21907707) »
  • Martin FTet al. Potential role of mesenchymal stem cells (MSCs) in the breast tumour microenvironment: stimulation of epithelial to mesenchymal transition (EMT). Breast Cancer Res Treat 124:317-26 (2010). WB; Human.Read more (PubMed: 20087650) »

See all 6 publications for this product

Publishing research using ab28028? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"