Vimentin antibody [Vim 3B4] (ab28028)

Product Name 

Vimentin antibody [Vim 3B4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Vim 3B4] to Vimentin

Immunogen 

Full length native protein (purified): Vimentin isolated from bovine eye lens.

Reacts with 
(species key)

Hu, Rat, Chk, Cow, Dog, Hm, Hrs, Rb, Xl

Specificity 

Ab28028 recognises Vimentin

Epitope 

Ab28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/200. The antibody can be used for labelling acetone fixed samples.
IHC-P: 1/100 - 1/200. Treat tissues with proteinase K or with heat mediated antigen retrieval for 20 min before commencing with IHC staining protocol, incubating the antibody for 30 min at room temperature. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
IHC-Fr: 1/100 - 1/200. The antibody can be used for labelling acetone fixed, frozen sections.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/200. Predicted molecular weight: 54 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human osteogenic sarcoma 4-998 cells for IP, cytoskeletal proteins from Xenopus laevis kidney epithelial cells and erythrocytes for WB.

Cellular localization 

Cytoplasmic

Research areas 

Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Vimentin
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Vim 3B4

Isotype 

IgG2a

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 15mM Sodium Azide.
Constituents: Cell culture supernatant dialysed against 50mM Tris HCl. pH 7.2.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

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Vimentin antibody [Vim 3B4] images:

References for Vimentin antibody [Vim 3B4] (ab28028)

  This product has been used in:

Bohn W et al. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201:1-7 (1992). PubMed: 1612114
If you publish research using ab28028 please let us know so that we can cite the reference on this datasheet.

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