Anti-Vimentin antibody (ab39376)
Key features and details
- Chicken polyclonal to Vimentin
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
Overview
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Product name
Anti-Vimentin antibody
See all Vimentin primary antibodies -
Description
Chicken polyclonal to Vimentin -
Host species
Chicken -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.
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Positive control
- ICC/IF: Mouse brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS
(10mM isotonic 0.9% w/v), pH 7.2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (5) |
1/100 - 1/200.
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Notes |
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ICC/IF
1/100 - 1/200. |
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 22352 Mouse
- SwissProt: P20152 Mouse
- Unigene: 268000 Mouse
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Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab39376 has been referenced in 8 publications.
- Treviño-Villarreal JH et al. Down-Regulation of a Profibrotic Transforming Growth Factor-β1/Cellular Communication Network Factor 2/Matrix Metalloprotease 9 Axis by Triamcinolone Improves Idiopathic Subglottic Stenosis. Am J Pathol 191:1412-1430 (2021). PubMed: 34111429
- Sinkey RG et al. Thrombin-Induced Decidual Colony-Stimulating Factor-2 Promotes Abruption-Related Preterm Birth by Weakening Fetal Membranes. Am J Pathol 190:388-399 (2020). PubMed: 31955792
- Mong EF et al. Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Sci Rep 10:3029 (2020). PubMed: 32080251
- Su N et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425
- Guzeloglu-Kayisli O et al. Mechanisms of chorioamnionitis-associated preterm birth: interleukin-1β inhibits progesterone receptor expression in decidual cells. J Pathol 237:423-34 (2015). PubMed: 26175191
- Mulholland DJ et al. Pten loss and RAS/MAPK activation cooperate to promote EMT and metastasis initiated from prostate cancer stem/progenitor cells. Cancer Res 72:1878-89 (2012). IHC . PubMed: 22350410
- Schilte C et al. Type I IFN controls chikungunya virus via its action on nonhematopoietic cells. J Exp Med 207:429-42 (2010). IHC-Fr ; Mouse . PubMed: 20123960
- Lawson DA et al. Basal epithelial stem cells are efficient targets for prostate cancer initiation. Proc Natl Acad Sci U S A 107:2610-5 (2010). ICC/IF ; Mouse . PubMed: 20133806