Overview
- Product nameAnti-Vimentin antibodySee all Vimentin primary antibodies ...
- DescriptionRabbit polyclonal to Vimentin
- Tested applicationsWB, IP more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Recombinant human protein purified from E.coli (His-Vimentin)
- Positive controlRD2, C6, HeLa and NIH3T3 cell lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride -
Concentration information loading... - Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab71144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
| IP | IP: Use at an assay dependent concentration. Use 4 µl for 200 µg lysate. |
Target
- FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. - Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
- Sequence similaritiesBelongs to the intermediate filament family.
- DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
- Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
- Unigene: 691131 Human
- Unigene: 268000 Mouse
- Unigene: 2710 Rat
see all
Target information above from: UniProt accession
P08670
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- FLJ36605 antibodyOTTHUMP00000019224 antibodyVIM antibody
- VIME_HUMAN antibodyvimentin antibody
see all
Anti-Vimentin antibody images
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Western blot - Vimentin antibody (ab71144)All lanes : Anti-Vimentin antibody (ab71144) at 1/5000 dilution
Lane 1 : RD2 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH3T3 cell lysate
Predicted band size : 54 kDa
Observed band size : 54 kDa
References for Anti-Vimentin antibody (ab71144)
This product has been referenced in:
- Lang BJ et al. Heat stress induces epithelial plasticity and cell migration independent of heat shock factor 1. Cell Stress Chaperones : (2012). Read more (PubMed: 22791010) »
- De Ceuleneer M et al. Quantification of citrullination by means of skewed isotope distribution pattern. J Proteome Res 11:5245-51 (2012). Read more (PubMed: 22978259) »
