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Product Name
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Vinculin antibody [V284]
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See all Vinculin antibodies (13)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [V284] to Vinculin
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Immunogen
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Vinculin derived from human platelets.
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Reacts with
(species key)
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Hu, Rb
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Specificity
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This antibody recognizes human vinculin.
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Tested applications
(see key)
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody may be diluted to a titer of 1:20-1:50 in an ABC method.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
. High temperature antigen unmasking of formalin-fixed paraffin embedded tissue sections must be performed prior to the immunostaining.
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Cellular localization
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Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.
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Research areas
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Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cardiovascular >> Heart >> Cardiac arrhythmias
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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V284
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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0.150 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vinculin antibody [V284] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Vinculin
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Vinculin antibody [V284] - more information
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