Anti-Vinculin antibody (ab61186)

Hi We use a lot of your NBR1 antibody listed below. I was wondering if there was a possibility of ordering a larger size? perhaps 100ug or 200ug instead of 50ug? If not, perhaps a quote if we order 3 lots in 1 go? BW  

ANSWER:

Thank you for your enquiry which has been forwarded to the scientific support team.

I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I can recommend to contact our customer service department with a completed  list of which antibodies and the quantity required for each product. They will be happy to then provide you with a quote. Please email: orders@abcam.com

Thank you for your interest in our products. If you have any further questions, please do not hesitate to contact us.

Hello,

We had purchased anti-vinculin antibody ab61186 from Abcam and we tested the antibody in IF on our human cardiac cells. Unfortunately we did not get the specific staining of vinculin in focal adhesions.

We used 2 positive controls in our test: anti-kindlin-2 antibodies (stains focal adhesions) and anti-vinculin antibody from Sigma.

Please find the pictures attached.

From our test we concluded that the antibodies we received are not specific for human vinculin.

We ask Abcam for reimbursement of the antibody costs.

ANSWER:

Thank you for contacting us and for reporting the problem you experienced with the antibody ab61186.

I am sorry that the product you received did not perform as stated expected. We have recently updated the datasheet and the antibody should indeed work for ICC/IF. As requested, I am happy to issue you a refund.

Since at Abcam we are concern about the quality of the products we supply, I would like to ask you further information regarding the samples you tested and the protocol you used. Could you please fill in the attached questionnaire? We will use those data to investigate the source of the problem with this product.

I look forward to receiving your reply and I apologize for the inconvenience this has caused. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.