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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab73412 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Vinculin antibody (ab73412)
All lanes : Anti-Vinculin antibody (ab73412) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 :
Lane 5 :
Lane 6 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 124 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes
Flow Cytometry - Vinculin antibody (ab73412)
ab73412 staining vinculin in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
Western blot - Anti-Vinculin antibody (ab73412)
Anti-Vinculin antibody (ab73412) at 1/1500 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique
Predicted band size : 124 kDa
Exposure time : 1 minute
Blocked with 5% milk for 1 hour at 22°C.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Anti-Vinculin antibody for Western blot in Human (73412)
Vinculin antibody for Flow Cytometry in Human (73412)
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