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Western blot - Vinculin antibody (ab73412)
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Product Name
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Vinculin antibody
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See all Vinculin antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Vinculin
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1100 to the C-terminus of Human Vinculin. (Peptide available as ab74076.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Chmp, Cow, Hrs, Pig, RMk, Zfsh, Zfnh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Lysates:
Mouse Kidney Tissue, EBS E14 TG2A Day 3 Whole Cell, HepG2 Whole Cell, Human Small Intestine Tissue, Human Skeletal Muscle Tissue, Rat Liver Tissue, Mouse Pancreas Tissue (data not shown)
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Cellular localization
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Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.
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Research areas
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Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cardiovascular >> Heart >> Cardiac arrhythmias
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vinculin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Vinculin antibody images, references, products related to ab73412 and other tools.
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Vinculin antibody images:
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Search PubMed (MEDLINE) for references to Vinculin
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Vinculin antibody - more information
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Customer reviews (feedback) regarding Vinculin antibody |
Customer FAQs regarding Vinculin antibody |
Protocols for Vinculin antibody |
Price and availability of products related to Vinculin antibody |
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Vinculin antibody - related products:
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Vinculin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Vinculin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - Vinculin antibody (ab73412)