Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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ab11194 has been referenced in 13 publications.
Publishing research using ab11194? Please let us know so that we can cite the reference in this datasheet
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ab11194 staining Vinculin in HUVEC cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.2% Triton X-100, blocked with 1% BSA for 16 hours at 4°C, then incubated with ab11194 at a 1/400 dilution for 1 hour at 22°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG polyclonal used at 5µg/ml.
Image courtesy of an anonymous Abreview.
ICC/IF image of ab11194 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11194, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
ab11194 staining vinculin in rat smooth muscle cells isolated from mesenteric artery cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20ºC. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4ºC. Alexa fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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