Anti-Vitamin D Binding protein antibody [5E8] (ab23484)

Overview

  • Product nameAnti-Vitamin D Binding protein antibody [5E8]
    See all Vitamin D Binding protein primary antibodies
  • Description
    Mouse monoclonal [5E8] to Vitamin D Binding protein
  • Specificityab23484 is specific for Gc-globulin.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Gc-globulin isolated from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab23484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB Use at an assay dependent dilution. Predicted molecular weight: 53 kDa.

Target

  • FunctionMultifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBP antibody
    • DBP/GC antibody
    • GC antibody
    • Gc globulin antibody
    • Gc-globulin antibody
    • GRD3 antibody
    • Group specific component antibody
    • Group specific component vitamin D binding protein antibody
    • Group-specific component antibody
    • hDBP antibody
    • VDB antibody
    • VDBG antibody
    • VDBP antibody
    • Vitamin D binding alpha globulin antibody
    • Vitamin D-binding protein antibody
    • VTDB_HUMAN antibody
    see all

Anti-Vitamin D Binding protein antibody [5E8] images

  • All lanes : Anti-Vitamin D Binding protein antibody [5E8] (ab23484) at 5 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 2 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
    Lane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Vitamin D Binding protein antibody [5E8] (ab23484)

This product has been referenced in:
  • Behrouz GF  et al. Presence of auto-antibody against two placental proteins, annexin A1 and vitamin D binding protein, in sera of women with pre-eclampsia. J Reprod Immunol 99:10-6 (2013). WB . Read more (PubMed: 23830177) »
  • Hernández-Martín A  et al. Synthesis of vitamin D3 analogues with A-ring modifications to directly measure vitamin D levels in biological samples. Bioorg Med Chem 21:7779-89 (2013). Read more (PubMed: 24216092) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

This particular lot of ab23484 was produced in October 2010.

Please let me know if there is anything else I can help you with.

Thank you for contacting Abcam.

We do not have expiration dates for our antibodies, but we guarantee them for 6 months after delivery. In general though they are stable for:

- 1 year at 4C
- 5 years at -20C
- 10 years at -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"