Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Vitamin D Receptor (phospho S208) antibody
See all Vitamin D Receptor products (15) ...
Description
Rabbit polyclonal to Vitamin D Receptor (phospho S208)
Specificity
Detects Vitamin D Receptor only when phosphorylated at serine 208.
Tested applications
WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide derived from human Vitamin D Receptor around the phosphorylation site of serine 208 (D-L-SP-E-E).
Positive control
A549 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Vitamin D Receptor
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Vitamin D Receptor
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
ELISA
ELISA: 1/10000ELISA: 1/10000
ICC/IF: 1/500 - 1/1000.
Target
Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP11473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Vitamin D3 receptor antibody
- 125 dihydroxyvitamin D3 receptor antibody
- Vitamin D3 receptor antibody
see all
Database links
- Entrez Gene: 7421 Human
- Omim: 601769 Human
- SwissProt: P11473 Human
- Unigene: 524368 Human
Anti-Vitamin D Receptor (phospho S208) antibody images:
Immunocytochemistry/ Immunofluorescence - Vitamin D Receptor (phospho S208) antibody (ab63572)
Immunofluorescent staining of A549 cells, using ab63572 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
Western blot - Vitamin D Receptor (phospho S208) antibody (ab63572)
developed using the ECL technique
Serum starved U937 cells were incubated with 0.5 µM H2O2, 10 µg/ml A24, or 3 µM DFO for 10 min at 37°C. Phosphorylation of VDR (P-VDR) in whole cell extracts was analyzed using ab63572.
©Callens et al., 2010. Originally published in The Journal of Experimental Medicine. doi: 10.1084/jem.20091488.
References for Anti-Vitamin D Receptor (phospho S208) antibody (ab63572)
This product has been referenced in:
- Callens Cet al. Targeting iron homeostasis induces cellular differentiation and synergizes with differentiating agents in acute myeloid leukemia. J Exp Med 207:731-50 (2010). WB; Human.Read more (PubMed: 20368581) »
See 1 publication for this product
Publishing research using ab63572? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"