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All lanes : Anti-Vitamin D Receptor (phospho S51) antibody (ab79311) at 1/500 dilution
Lane 1 : COLO cells extract treated with insulin (0.01U/ml, 15mins)
Lane 2 : COLO cells extract treated with insulin (0.01U/ml, 15mins) with Immunizing phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 48 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
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