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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Vitamin D Receptor (phospho S51) antibody
See all Vitamin D Receptor products (15) ...
Description
Rabbit polyclonal to Vitamin D Receptor (phospho S51)
Specificity
ab79311 detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human Vitamin D3 Receptor around the phosphorylation site of serine 51 (R-R-SP-M-K).
Positive control
COLO cell extract treated with insulin (0.01U/ml for 15mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Vitamin D Receptor
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Vitamin D Receptor
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Western blot - Vitamin D Receptor (phospho S51) antibody (ab79311)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 38 kDa (predicted molecular weight: 48 kDa).
ELISA
ELISA: 1/20,000ELISA: 1/20,000
Target
Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP11473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Vitamin D3 receptor antibody
- 125 dihydroxyvitamin D3 receptor antibody
- Vitamin D3 receptor antibody
see all
Database links
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all
Anti-Vitamin D Receptor (phospho S51) antibody images:
Western blot - Vitamin D Receptor (phospho S51) antibody (ab79311)
All lanes : Anti-Vitamin D Receptor (phospho S51) antibody (ab79311) at 1/500 dilution
Lane 1 : COLO cells extract treated with insulin (0.01U/ml, 15mins)
Lane 2 : COLO cells extract treated with insulin (0.01U/ml, 15mins) with Immunizing phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 48 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
References for Anti-Vitamin D Receptor (phospho S51) antibody (ab79311)
ab79311 has not yet been referenced specifically in any publications.
Publishing research using ab79311? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"