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Overview

  • Product nameAnti-Vitronectin antibody [BV1]See all Vitronectin primary antibodies ...
  • Description
    Mouse monoclonal [BV1] to Vitronectin
  • SpecificityThe antibody binds to soluble vitronectin as well as to membrane bound vitronectin.
  • Tested applicationsIHC-P, WB, IP, ICC/IF, Purification more details
  • Species reactivity
    Reacts with: Human

Properties

Applications

Our Abpromise guarantee covers the use of ab7165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent concentration. PubMed: 19730772
WB WB: Use at an assay dependent concentration.
IP IP: Use at an assay dependent concentration.
ICC/IF ICC/IF: Use at an assay dependent concentration.
Purification P: Use at an assay dependent concentration.

Target

  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin-like domains.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted > extracellular space.

    • Target information above from: UniProt accession P04004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Complement S Protein antibodyEpibolin antibodyS Protein antibody
        S-protein antibodySerum Spreading Factor antibodySerum-spreading factor antibodySomatomedin B antibodySomatomedin-B antibodyV75 antibodyVitronectin V10 subunit antibodyVitronectin V65 subunit antibodyVN antibodyVNT antibodyVTN antibodyVTNC_HUMAN antibody
      see all

    References for Anti-Vitronectin antibody [BV1] (ab7165)

    This product has been referenced in:
    • Knappe UJ  et al. Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater. Acta Neurochir (Wien) 152:345-53 (2010). IHC-P ; Human . Read more (PubMed: 19730772) »

    See 1 Publication for this product

    Publishing research using ab7165 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab7165 Submit a Question for ab7165

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Thanks for the response. We are sure about the quantity of vitronectin in the sample because the smaple serum was enriched for the experiment. I used 1:200 dilution and incubated more than 2 hrs. Usually any antibody gives some signal at this dilution. A...

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    We regret to hear about your problems with ab7165 monoclonal antibody to human vitronectin. The antibody is described in the following article: Zanetti, A et al; Clustering of vitronectin and RGD peptides on microspheres leads to engagement of integri...

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    Follow up to previous enquiry: Thanks for the response. We are sure about the quantity of vitronectin in the sample because the smaple serum was enriched for the experiment. I used 1:200 dilution and incubated more than 2 hrs. Usually any antibody giv...

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    I have contacted the source of the antibody to find out if they can recommend a protocol. Could you please confirm you used protease inhibitors and kept the samples cold at all times during your experiment prior to boiling your samples with loading bu...

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    BATCH NUMBER 74939 ORDER NUMBER 58763 DESCRIPTION OF THE PROBLEM No signal or weak signal There was no signal in blots (2 seperate blots) SAMPLE Human serum, purified, 125 ug /blot PRIMARY ANTIBODY Abcam, ab7165-50, Mouse monoc...

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    I'm sorry you are having problems with ab7165 and would like to thank you for taking the time to give me details of your protocol, it is very useful. I would like to suggest incubating overnight at 4C and try also more concentrated antibody. Could...

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    Since we are interested in detecting the beta subunit of the vitronectin receptor (alpha v, beta 3), we would like to know: a) which integrin subunit your monoclonal antibody for vitronectin is able to recognize? b) whether the same antibody cross-react...

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    The subunit recognise by this antibody is undetermined, as is it's cross-reactivity with mouse.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"