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Anti-Vitronectin antibody [EP873Y] (ab45139) at 1/20000 dilution + Hu Serum membrane at 10 µg
Secondary
Goat anti-rabbit HRP labelled, at 1/2000 dilution
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Ab45139 at a 1/100 dilution, staining human Vitronectin in human liver tissue by immunohistochemistry using paraffin embedded tissue.
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