![Western blot - Vitronectin antibody [EP873Y] (ab45139) image](#ab45139_1.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - Vitronectin antibody [EP873Y] (ab45139) image](#ab45139_2.bmp)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Vitronectin antibody [EP873Y]
See all Vitronectin products (12) ...
Description
Rabbit monoclonal [EP873Y] to Vitronectin
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the V10 subunit of human Vitronectin.
Positive control
Human liver tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP873Y
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> Complement >> Regulatory
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/20000Detects a band of approximately 75 kDa.
IHC-P: 1/100 - 1/250.(Antigen retrieval is recommended.)
ICC/IF
ICC/IF: 1/50 - 1/100.ICC/IF: 1/50 - 1/100.
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Tissue specificity
Plasma.
Sequence similarities
Contains 4 hemopexin-like domains.
Contains 1 SMB (somatomedin-B) domain.
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
Post-translational
modifications
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP04004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
Database links
- Entrez Gene: 7448 Human
- Entrez Gene: 22370 Mouse
- Entrez Gene: 29169 Rat
- Omim: 193190 Human
- SwissProt: P04004 Human
- SwissProt: P29788 Mouse
- SwissProt: Q62905 Rat
- Unigene: 2257 Human
see all
Anti-Vitronectin antibody [EP873Y] images:
Western blot - Vitronectin antibody [EP873Y] (ab45139)
![Western blot - Vitronectin antibody [EP873Y] (ab45139)](/ps/datasheet/Images/45/ab45139/ab45139_1.bmp)
Anti-Vitronectin antibody [EP873Y] (ab45139) at 1/20000 dilution + Hu Serum membrane at 10 µg
Secondary
Goat anti-rabbit HRP labelled, at 1/2000 dilution
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Vitronectin antibody [EP873Y] (ab45139)
![Immunohistochemistry (Paraffin-embedded sections) - Vitronectin antibody [EP873Y] (ab45139)](/ps/datasheet/Images/45/ab45139/ab45139_2.bmp)
Ab45139 at a 1/100 dilution, staining human Vitronectin in human liver tissue by immunohistochemistry using paraffin embedded tissue.
References for Anti-Vitronectin antibody [EP873Y] (ab45139)
ab45139 has not yet been referenced specifically in any publications.
Publishing research using ab45139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"