Vitronectin antibody [VIT-2] (ab11591)

Product Name 

Vitronectin antibody [VIT-2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [VIT-2] to Vitronectin

Immunogen 

Full length native protein (purified) (Human).

Reacts with 
(species key)

Hu

Specificity 

Monoclonal Anti-Vitronectin recognizes both the 75 kDa and 65 kDa bands of human purified and plasma vitronectin in an immunoblotting assay.
It does not crossreact with other extracellular matrix components such as fibronectin, laminin, merosin, collagen IV or chondroitin sulfate A, B and C in dot blot assays.

Tested applications 
(see key)

Dot, ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
WB: 1/1500 (see Abreview). Detects bands of approximately 75 and 65 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted, Extracellular

Research areas 

Immunology >> Innate Immunity >> Complement >> Regulatory
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

VIT-2

Isotype 

IgM

Purity 

Ascites

Storage buffer 

Preservative: 0.1% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

See below for Vitronectin antibody [VIT-2] images, references, products related to ab11591 and other tools.

Vitronectin antibody [VIT-2] images:

References for Vitronectin antibody [VIT-2] (ab11591)

  This product has been used in:

Blumenstein M et al. Aberrant Processing of Plasma Vitronectin and High-Molecular-Weight Kininogen Precedes the Onset of Preeclampsia. Reprod Sci 16:1144-1152 (2009). PubMed: 19657137

If you publish research using ab11591 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Vitronectin antibody [VIT-2]

Customer FAQs regarding Vitronectin antibody [VIT-2]

Protocols for Vitronectin antibody [VIT-2]

Price and availability of products related to Vitronectin antibody [VIT-2]

Vitronectin antibody [VIT-2] - related products: