You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Vitronectin antibody [VIT-2] (ab11591)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Vitronectin antibody [VIT-2]
See all Vitronectin products (12) ...

Description

Mouse monoclonal [VIT-2] to Vitronectin

Specificity

Monoclonal Anti-Vitronectin recognizes both the 75 kDa and 65 kDa bands of human purified and plasma vitronectin in an immunoblotting assay. It does not crossreact with other extracellular matrix components such as fibronectin, laminin, merosin, collagen IV or chondroitin sulfate A, B and C in dot blot assays.

Tested applications

IHC-P, ELISA, WB, Dot Blot, ICCmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length native protein (purified) (Human).

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

VIT-2

Isotype

IgM

Research areas

Immunology >> Innate Immunity >> Complement >> Regulatory
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - Vitronectin antibody [VIT-2] (ab11591)Western blot - Vitronectin antibody [VIT-2] (ab11591) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

Tissue specificity

Plasma.

Sequence similarities

Contains 4 hemopexin-like domains.
Contains 1 SMB (somatomedin-B) domain.

Domain

The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.

Post-translational
modifications

Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracelllular medium.

Cellular localization

Secreted > extracellular space.

Target information above from: UniProt accessionP04004 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Complement S Protein antibody
  • Epibolin antibody
  • S Protein antibody
  • S-protein antibody
  • Serum Spreading Factor antibody
  • Serum-spreading factor antibody
  • Somatomedin B antibody
  • Somatomedin-B antibody
  • V75 antibody
  • Vitronectin V10 subunit antibody
  • Vitronectin V65 subunit antibody
  • VN antibody
  • VNT antibody
  • VTN antibody
  • VTNC_HUMAN antibody
see all

Database links

Anti-Vitronectin antibody [VIT-2] images:

  Western blot - Vitronectin antibody [VIT-2] (ab11591)

Western blot - Vitronectin antibody [VIT-2] (ab11591)

All lanes : Anti-Vitronectin antibody [VIT-2] (ab11591) at 1/1500 dilution

Lane 1 : 25ul Human csf 3 days post injury
Lane 2 : 25ul Human csf 4 days post injury
Lane 3 : 25ul Human csf 5 days post injury
Lane 4 : 25ul Human csf 6 days post injury
Lane 5 : 0.365ug recombinant vitronectin

Secondary
HRP conjugated sheep anti-mouse IgG
developed using the ECL technique

Performed under non-reducing conditions.

Observed band size : 65,75 kDa (why is the actual band size different from the predicted?)


Exposure time : 2 minutes

This image is courtesy of an Abreview submitted by Dr Anki Nordqvist

See Abreview

References for Anti-Vitronectin antibody [VIT-2] (ab11591)

This product has been referenced in:

  • Burton VJet al. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture. Exp Cell Res 317:276-92 (2011). ELISA; Human.Read more (PubMed: 21056557) »
  • Echevarria TJet al. Vitronectin: a matrix support factor for human limbal epithelial progenitor cells. Invest Ophthalmol Vis Sci 52:8138-47 (2011).Read more (PubMed: 21896866) »

See all 5 publications for this product

Publishing research using ab11591? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"