Vitronectin antibody [VN58-1] (ab13413)

Product Name 

Vitronectin antibody [VN58-1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [VN58-1] to Vitronectin

Immunogen 

Human Vitronectin.

Reacts with 

Hu

Does not react with 
(species key)

Cow

Specificity 

This antibody does not interfere with vitronectin-mediated adhesion.

Epitope 

This antibody specifically reacts with an epitope in the region of amino acids 1-130 of human vitronectin.

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/3000, with solid phase antigen.
IHC-P: Use at a concentration of 5 - 10 µg/ml.
IHC-Fr: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 5 - 10 µg/ml, under reducing and non reducing conditions. Predicted molecular weight: 54 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted, Extracellular

Research areas 

Immunology >> Innate Immunity >> Complement >> Regulatory
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

VN58-1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Vitronectin antibody [VN58-1] images, references, products related to ab13413 and other tools.

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References for Vitronectin antibody [VN58-1] (ab13413)

  This product has been used in: (two most recent references)

Gerold G et al. A Toll-like receptor 2-integrin beta3 complex senses bacterial lipopeptides via vitronectin. Nat Immunol 9:761-8 (2008). PubMed: 18516040

Vallon M & Essler M Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans. J Biol Chem 281:34179-88 (2006). PubMed: 16982628
If you publish research using ab13413 please let us know so that we can cite the reference on this datasheet.

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