![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin antibody [VN58-1] (ab13413) image](#ab13413_1.jpg)
 image](#Vitronectin-Primary-antibodies-ab13413-1.jpg)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Vitronectin antibody [VN58-1]
See all Vitronectin products (12) ...
Description
Mouse monoclonal [VN58-1] to Vitronectin
Specificity
This antibody does not interfere with vitronectin-mediated adhesion.
Tested applications
ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Does not react with
Cow
Immunogen
Human Vitronectin.
Epitope
This antibody specifically reacts with an epitope in the region of amino acids 1-130 of human vitronectin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
VN58-1
Isotype
IgG1
Research areas
Immunology >> Innate Immunity >> Complement >> Regulatory
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
ELISA: 1/3000((with solid phase antigen))
WB: Use a concentration of 5 - 10 µg/ml.Predicted molecular weight: 54 kDa.
IHC-P: Use a concentration of 5 - 10 µg/ml.
IHC-Fr: Use a concentration of 5 - 10 µg/ml.
Target
Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Tissue specificity
Plasma.
Sequence similarities
Contains 4 hemopexin-like domains.
Contains 1 SMB (somatomedin-B) domain.
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
Post-translational
modifications
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP04004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
Database links
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
Anti-Vitronectin antibody [VN58-1] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin antibody [VN58-1] (ab13413)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin antibody [VN58-1] (ab13413)](/ps/datasheet/Images/13/ab13413/ab13413_1.jpg)
This picture shows formalin-fixed paraffin embedded human skin stained with Vitronectin (1:100 - 30 minutes RT). The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
Immunocytochemistry/ Immunofluorescence-Vitronectin antibody [VN58-1](ab13413)
](/ps/datasheet/images/13/ab13413/Vitronectin-Primary-antibodies-ab13413-1.jpg)
ICC/IF image of ab13413 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13413, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Vitronectin antibody [VN58-1] (ab13413)
This product has been referenced in:
- Stepanek Oet al. Interaction of late apoptotic and necrotic cells with vitronectin. PLoS One 6:e19243 (2011).Read more (PubMed: 21573223) »
- Hicks BGet al. Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications. Biomaterials : (2010). IHC-P; Human.Read more (PubMed: 21030076) »
See all 4 publications for this product
Publishing research using ab13413? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"