You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Vitronectin protein (Biotin) (Human) (ab92639)

Submit an Abreview Q&A (1)

Overview

  • Product nameVitronectin protein (Biotin) (Human)See all Vitronectin proteins and peptides ...
  • Protein descriptionFull length native Human Vitronectin from Human plasma, MWt 75kDa. UniProt ID = P04004.
  • Expression hostNative
  • ConjugationBiotin

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab92639 is prepared from fresh Human plasma using non-denaturing chromatography, then biotin labeled at primary amines. Purity is > 98% pure by SDS-PAGE.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Conjugation notesLabeling ratio varies lot-to-lot
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92639 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Complement S ProteinEpibolinS Protein
        S-proteinSerum Spreading FactorSerum-spreading factorSomatomedin BSomatomedin-BV75Vitronectin V10 subunitVitronectin V65 subunitVNVNTVTNVTNC_HUMAN
      see all
  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin-like domains.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted > extracellular space.

    • Target information above from: UniProt accession P04004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Vitronectin protein (Biotin) (Human) images

    • SDS-PAGE - Vitronectin protein (Biotin) (Human) (ab92639)
      SDS-PAGE - Vitronectin protein (Biotin) (Human) (ab92639)
      10% SDS-PAGE
      Lane 1: Vitronectin (2 ug) Reduced
      Lane 2: Vitronectin (2 ug) Non-Reduced
      Lane 3: Prestained Standard
    • Western blot - Vitronectin protein (Biotin) (Human) (ab92639)
      Western blot - Vitronectin protein (Biotin) (Human) (ab92639)
      All lanes : anti-Vitronectin Avidin-AP at 1/3000 dilution

      Lane 1 : ab92639 (Reduced)
      Lane 2 : ab92639 (Non-reduced)

      Lysates/proteins at 2 µg per lane.


      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References for Vitronectin protein (Biotin) (Human) (ab92639)

    ab92639 has not yet been referenced specifically in any publications.

    Publishing research using ab92639 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab92639 Submit a Question for ab92639

    Displaying 1 - 1 of 1 results for Abreviews and Q&A

    10
    Votes: Highest First

    Hi, I have a question about the Vitronectin protein (Biotin) (Human) product. In "conjugation notes", you wrote that there are 2 moles biotin / mole Vitronectin. I wonder how this was determined, and if this ratio is accurate enough to perform q...

    Read More

    Thank you for contacting us with your question, and for your patience while I have been in touch with the lab for more information about this protein.
    I apologize but the information on our datasheet is incorrect. The labeling ratio actually varies l...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"