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Recombinant fragment, corresponding to amino acids 1-154 of Human Von Hippel Lindau protein with a His tag, purified from E. coli.
Our Abpromise guarantee covers the use of ab115496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. Ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. Predicted molecular weight: 24 kDa.|
|IHC-P||Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
ab115496 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"